Sensitivity of human gastric cancer cells to TRAIL is not associated with caspase-8 promoter methylation status
- VernacularTitle:胃癌细胞对TRAIL的敏感性与caspase-8基因甲基化状态的关系
- Author:
Rugang ZHANG
;
Dianchun FANG
;
Liuqin YANG
;
Yuanhui LUO
;
- Publication Type:Journal Article
- Keywords:
Aza CdR;
TRAIL;
caspase 8 methylation;
gastric carcinoma
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of methylation state of C5 of the cytosine in the CpG di nucleotide of caspase 8 promoter on tumor necrosis factor related apoptosis inducing ligand (TRAIL) antitumor activity in gastric carcinomas. Methods The methylation states of the caspase 8 promoter region of 5 kinds of gastric carcinoma strains were measured by methylation specific PCR method. The antitumor activity of TRAIL protein was measured by MTT method. Results No methylation of caspase 8 promoter was found in gastric carcinoma cells. Treatment with demethylation agent 5 Aza 2′ deoxycytidine (5 Aza CdR) increased sensitivity of gastric cancer cells to TRAIL, but did not change methylation status of caspase 8 promoter in gastric carcinoma cells. Conclusion Caspase 8 promoter methylation status is not associated with TRAIL antitumor activity.
