Effects of nitric oxide on NCAM production during LTP induction in hippocampal slices
- VernacularTitle:海马脑片LTP产生中NO对NCAM蛋白合成的影响
- Author:
Zhian HU
;
Baicheng CHEN
;
Haidi LI
;
Jun LUO
;
- Publication Type:Journal Article
- Keywords:
long tern potentiation;
nitric oxide;
neural cell adhesion molecule;
hippocampus;
rat
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of NMDA receptors and nitric oxide (NO) on neural cell adhesion molecule(NCAM) synthesis during hippocampal long term potentiation (LTP) induction in vitro . Methods LTP induction and maintenance were tested by using extracelluar electrophysiological recording. The synthesis of NCAM protein was detected by Western blotting. Results Application of conditioned stimulation induced LTP and a rise in NCAM at 10 min. NCAM protein level continued to rise while LTP remained stably at 60 min. The NMDA receptor inhibitor AP 5 and the NO synthase inhibitor N nitro arginine inhibited the LTP induction and the increase in the NCAM synthesis. Conclusion The changes in NCAM synthesis during hippocampal LTP induction in vitro may be involved in NMDA receptors and NO.
