Application of fluorescence recovery after photobleaching in the detection of gap junctional intercellular communication in bladder smooth muscle
- VernacularTitle:荧光光漂白恢复技术在检测膀胱平滑肌细胞间通讯功能中的应用
- Author:
Fenghai ZHOU
;
Bo SONG
;
Xiyu JIN
;
Wei SUN
;
- Publication Type:Journal Article
- Keywords:
fluorescence recovery after photobleaching;
laser scanning confocal microscope;
bladder smooth muscle
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the functional changes of gap junctional intercellular communication(GJIC) in bladder smooth muscle. Methods The functions of GJIC in bladder smooth muscle were detected by fluorescence recovery after photobleaching(FRAP). The mean fluorescence recovery rates of the bladder smooth muscle cells in the experimental group and the control group were compared. Results The mean fluorescence recovery rate of the experimental group was significantly higher than that in the control group( P
