Demethylation of progesterone receptor in leukemia cell line induced by DNMT1 antisense oligonucleotide wrapped in liposome
- VernacularTitle:脂质体包裹DNMT1反义寡核苷酸诱导白血病细胞株孕酮受体去甲基化的实验研究
- Author:
Xiaobing ZHANG
;
Zejun LIU
;
- Publication Type:Journal Article
- Keywords:
DNMT1;
antisense oligonucleotide;
leukemia;
progesterone receptor;
methylation;
liposomes
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the transfection of DNMT1 antisense oligonucleotide wrapped in liposome on the promotor methylation and expression of progesterone receptor in leukemia cell lines so as to find a new pathway of methylation for gene therapy. Methods Artificially synthesized DNMT1 antisense oligonucleotide MG88 wrapped in liposome was transfected into the leukemia cell line. Changes of DNMT1 & PRB mRNA were detected by RT PCR, and the changes of the methylation status of PRA & PRB were analyzed by MSP. Results After transfection of leukemia cell line with MG88, DNMT1 mRNA decreased significantly. PRA & PRB promotors were demethylated, and the expressions of PRB mRNA increased significantly. Conclusion MG88 can induce the demethylation of PR promoters in leukemia cell line, resulting in the increase of PRB mRNA expression. So MG88 can be used in the experimental study of DNA methylation for gene therapy.
