Biocompatibility of PDLLA/HA/DBM with human osteoblasts co-cultured in vitro
- VernacularTitle:消旋聚乳酸/羟基磷灰石/脱钙骨基质材料支架的体外细胞相容性实验研究
- Author:
Qiaonan GUO
;
Jianhua ZHAO
;
Jiayou LU
;
- Publication Type:Journal Article
- Keywords:
osteoblast;
biocompatibility;
cell culture;
PDLLA/HA/DBM
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the osteocompatibility of poly(D,L lactic acid)/ hydroxyapatite/decalcified bone matrix (PDLLA/HA/DBM) and to compare the osteocompatibility with that of PDLLA and DBM. Methods The isolated human osteoblasts from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA, and DBM. The interface between biomaterial and osteoblasts was investigated with phase contrast microscope and scanning electron microscope. Results Osteoblasts were attached tightly to the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrix covered the surface of biomaterial and packed the granules of biomaterial completely. Conclusion PDLLA/HA/DBM can form osteointegrated interface at the early stage with good biocompability.
