Analysis of microsatellite instability at BAT-26 locus in esophageal cancer
- VernacularTitle:食管癌BAT-26位点微卫星不稳的分析
- Author:
Kun YAN
;
Mingrong WANG
;
Dianchun FANG
;
Kang YANG
;
- Publication Type:Journal Article
- Keywords:
microsatellite;
microsatellite instability;
BAT 26;
monomorphism
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the microsatellite instability(MSI) and monomorphism at microsatellite DNA BAT 26 locus in esophageal mucosa. Methods Genomic DNA extracted from tissues was amplified by PCR. PCR products were run on 9% denaturing polyacrylamidegel and stained with silver. Then MSI and monomorphism of microsatellite at BAT 26 locus in normal tissues at the incised edge and esophageal cancer tissues in 45 cases of esophageal caner were analyzed. Results All normal specimens showed no change in the length of MS DNA at BAT 26 locus. MSI was detected in 3 out of 45(6 7%) cases of esophageal cancer. Conclusion BAT 26 has complete monomorphism in genome of normal esophageal tissues, but is not sensitive to the identification of MSI.
