Changes of expression levels of 12S rRNA and COXⅠ mRNA encoded by mtDNA in rat brain during hypoxic exposure
- VernacularTitle:缺氧过程中大鼠脑mtDNA编码12S rRNA和COXⅠ mRNA表达的变化
- Author:
Junze LIU
;
Xiaoling TAN
;
Lifei CAO
;
- Publication Type:Journal Article
- Keywords:
hypoxic exposure;
mtDNA expression;
12S rRNA;
cytochrome oxidase subunitⅠ mRNA
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of expression levels of 12S rRNA and cytochrome oxidase subunit Ⅰ (COXⅠ) mRNA encoded by mtDNA in rat cerebral cortex after rat exposure to hypobaric hypoxia for different days. Methods Healthy male Wistar rats were exposed to hypobaric chamber simulating 5 000 m above sea level (23 5 h/day) for 2, 5, 15 and 30 d. Rats in the control group were not exposed to hypoxia. Rats were sacrificed by decapitation. Total RNA in cerebral cortex was extracted using a standard program. Transcriptional levels of 12S rRNA and COXⅠ mRNA were determined by reverse transcription polymerase chain reaction (RT PCR). Results Compared with that in the control, the expression of 12S rRNA increased by 57% after hypoxic exposure for 2 d ( P 0 05). Compared with that in the control group, the expression of COXⅠ mRNA increased significantly by 55% and 106% after hypoxic exposure for 2 and 5 d ( P 0 05). Conclusion Hypoxic exposure may have effect on both protein gene and ribosome gene expression encoded by mtDNA, and the expression changes in a hypoxic exposure time dependent manner. This suggests that hypoxia can have effect on mitochondrial oxidative phosphorylation gene expression at both mitochondrial transcriptional and translational levels.
