Effects of TSPG combined with EPO or GM-CSF on differentiation of K562 cells
- VernacularTitle:人参皂甙协同EPO和GM-CSF对K562细胞诱导分化的实验研究
- Author:
Dilong CHEN
;
Yaping WANG
;
Tingmei CHEN
;
- Publication Type:Journal Article
- Keywords:
saponins of Panax ginseng;
cytokine;
K562;
differentiation
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of total saponins of Panax ginseng(TSPG) combined with erythropoietin(EPO) or granulocyte macrophage colony stimulating factor(GM CSF) on the differentiation of K562 cells. Methods Cells were cultured in vitro . Morphologic changes of cells were observed with light microscope and electron microscope. The levels of hemoglobin, peroxidase, non specific lipase ? NAE in cells were detected respectively by benzidine staining, peroxidase(POX) staining and ? NAE cytochemical staining. Levels of cell surface differentiation antigens CD15, HIR2, EPO R and GM CSF R were determined by immunocytochemistry. Results TSPG combined with EPO or GM CSF resulted in more mature morphological features of K562, increased expressions of hemoglobin and myeolperoxidase in K562 cells, and enhanced expressions of cell surface differentiation antigens CD15, HIR2, EPO R and GM CSF R. Conclusion TSPG combined with cytokine EPO or GM CSF can induce more mature differentiation of K562 cells.
