EXPRESSION OF UBIQUITIN mRNA IN MYOCARDIUM OF BURN RATS WITH SEPSIS
- VernacularTitle:烧伤脓毒症早期心肌内泛素mRNA的表达变化及意义
- Author:
Chuanan SHEN
;
Jiake CHAI
;
Zhiyong SHENG
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
burns;
sepsis;
myocardium;
ubiquitin;
blotting, Northern
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein metabolism in rat myocardium after burn injury with sepsis, the mRNA expressions of ubiquitin were determined. Methods Wistar rats were subjected to a 30% full thickness burn and endotoxin (6mg/kg) was immediately injected into the peritoneal cavity. They were randomly divided into 2 and 6 hour groups and a normal control group, with 9 rats in each group. The cardiac muscle was taken to assay the mRNA expressions of ubiquitin during postinjury period. Results The expressions of the ubiquitin mRNA (2 4kb and 1 2kb), especially the 2 4kb stripe, in cardiac muscle of burn sepsis rats were significantly higher than that of normal control ( P
