THE EFFECT OF UBIQUITIN-PROTEASOME PATHWAY ON NUCLEAR FACTOR KAPPA B ACTIVITY IN LIVER OF BURN RATS WITH SEPSIS
- VernacularTitle:泛素-蛋白酶体途径在烧伤脓毒症大鼠肝脏NF-?B活化中的作用研究
- Author:
Huinan YIN
;
Jiake CHAI
;
Yanqiu WU
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
sepsis;
ubiquitin;
NF kappa B;
inhibitory ?B ?(I?B ?);
tumor necrosis factor;
liver
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objevtive To study the effect of ubiquitin proteasome pathway inhibition on NF ?B activity and I?B?expression as well as TNF ? secretion in the liver in burned rats with sepsis. Methods Rats were subjected to 30% full thickness scald injury, followed by intraperitoneal injection of lipopolysaccharide (LPS), was used for this experiment to mimic early sepsis after burn. Sixty Wistar rats were randomly divided into normal control group, burn sepsis group, burn sepsis with proteasome inhibitor N Acetyl leucinyl leucinyl norleucinal (ALLN) treatment group, and burn sepsis with NF ?B inhibitor Pyrrolidine Dithiocarbamate (PDTC) treatment group with 6 rats in each group. The following parameters were measured with the method indicated in parentheses: NF ?B activity (electrophoretic mobility shift assay, EMSA), I?B ? expression (Western blotting), and TNF ? (Enzyme linked immunoadsordent assay, ELISA). Results These showed that NF ?B activity was markedly activated and reached its peak 1h after challenge ( P
