THE EFFECT OF DIFFERENT CONCENTRATIONS OF CYSTEINE NO AND ET PRODUCTION BY CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
- VernacularTitle:不同浓度同型半胱氨酸对培养的人脐静脉内皮细胞分泌一氧化氮及内皮素的影响
- Author:
Litao RUAN
;
Tiesheng CAO
;
Yunyou DUAN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
homocysteine;
endothelin;
nitric oxides;
umblilical vein endothelial cell
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of different concentrations of homocysteine (Hcy) on production of nitric oxide (NO) and endothelin (ET) by cultured human umbilical vein endothelial cells (HUVEC). Methods HUVECs were collected and cultured to the third passage. Then HUVECs were co cultured with Hcy in different concentrations for 24 hours. The contents of NO and ET in culture medium were examined at 4,6,8,24 hours. Results After HUVECs were exposed to different concentrations of Hcy for 24 hours, the content of NO in culture medium was significantly reduced compared with the control group ( P
