EFFECTS OF IL-8 AND IFN-? ON CULTURED ECTOPIC ENDOMETRIAL CELLS
- VernacularTitle:白介素8和干扰素?对离体培养的子宫内膜细胞的影响
- Author:
Jie LI
;
Weiguo ZHENG
;
Xiaoyan XIN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
endometriosis;
cell culture;
interleukin 8;
interferon alpha;
ultrastructure
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to investigate the effects of interleukin 8 (IL 8) and interferon (IFN) alpha on proliferation of ectopic endometrial cells in vitro . Methods Ectopic endometrial cells from women suffering from endometriosis were cultured, purified, and identified by immunocytochemistry. Cultured endometrial cells were treated with IL 8 and IFN ? 2b in various concentrations in 96 well plates (IL 8 in 0 1ng/ml, 1ng/ml, 5ng/ml, 10ng/ml, 50ng/ml, IFN ? in 10U/ml, 50U/ml, 100U/ml, 1 000U/ml, 10 000U/ml). The effects of IL 8 and IFN ? 2b on endometrial cell proliferation were assessed using MTT. The ultrastructural changes in endometrial cells, which were incubated in medium containing 1ng/ml of IL 8, were observed with transmission electron microscopy. Results The data suggested that there was a dose dependent stimulatory effect of IL 8 on cell proliferation. IL 8 could stimulate proliferation of endometrial cells, and the most effective concentration was 10ng/ml ( P
