Properties of the binding of LPS to vascular endothelial cells
- VernacularTitle:内毒素与血管内皮细胞结合特性初探
- Author:
Jian CHEN
;
Xiang WANG
;
Xiangdong LUO
;
Zongcheng YANG
;
- Publication Type:Journal Article
- Keywords:
endotoxin;
vascular endothelial cell
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the properties of the serum free binding of vascular endothelial cells(ECs) to lipopolysaccharide (LPS). Methods The binding of ECs to fluorescein isothiocyanate labelled LPS(FITC LPS) was observed with laser confocal microscope(LCM). The extract of ECs membrane after binding with FITC LPS was recorded with fluorescence microscope. Results The binding of ECs to FITC LPS was in a dose dependent manner. The fluorescence was mainly distributed in cytoplasm, especially near the nucleus which could also be stained. The bound FITC LPS had a very close relationship with the membranous system of ECs. Conclusion ECs could bind to LPS directly and could function intracellularly.
