Protective effects of prostaglandin E_1 on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells
- VernacularTitle:PGE_1对培养乳鼠心肌细胞缺氧/复氧损伤的保护作用
- Author:
Lijie SONG
;
Runfang FU
;
Zhenji WANG
;
Guoqing FENG
;
Shiai WENG
;
- Publication Type:Journal Article
- Keywords:
prostaglandin E 1;
culture;
myocardial cell;
hypoxia/reoxygenation
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the protective effects of prostaglandin E 1 on hypoxia/reoxygenation injury in cultured neonatal rat myocytes. METHODS The hypoxia/reoxygenation injury model of cultured neonatal rat myocardial cells was developed. The activities of plasma superoxide dismutase (SOD) were measured by the method of xanthine oxidase and the contents of serum malonicaldehyde (MDA) were determined by the method of thiobarbituric acid. The activities of dehydrogenase in mitochondria were detected by MTT assay. The activities of LDH in culture were also evaluated. CONCLUSION PGE 1 has protective effects on the cultured neonatal rat myocardial cells injured by hypoxia/reoxygenation. The mechanisms are related to its antioxidation effects.
