Spindle poisons induce tk gene mutation in mouse lymphoma cells
- VernacularTitle:纺锤体毒剂诱导小鼠淋巴瘤细胞tk基因突变的研究
- Author:
Jian YUAN
;
Shengxue LIU
;
Jia CAO
;
- Publication Type:Journal Article
- Keywords:
vincristine;
colchicine;
spindle poisons;
tk gene;
mutagenesis;
L5178Y cell
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of spindle poisons (Vincristine, VCR and Colchicine, COL) on the tk gene of mouse lymphoma cells. Methods L5178Y cells were treated with VCR and COL at different concentrations. Determination of cytotoxicity, cell inoculation efficiency, relative survival rate, relative suspension growth rate and mutation frequency was performed. Results The relative survival rate and suspension growth rate of L5178Y cells decreased significantly with the increasing doses of VCR and COL. The mutation frequency of tk gene induced by VCR (0.5～2.5 ng/ml) and COL(10～50 ng/ml) was 1～3 times higher than that of spontaneous mutation frequency of L5178Y cells. Conclusion VCR and COL have obvious cytotoxic and mutagenic effects on tk gene in L5178Y cells.
