Screening of the protein interacting with inhibitor of differentiation 1′ using yeast two hybrid method
- VernacularTitle:酵母双杂交筛选与分化抑制因子Id1′相互作用的蛋白
- Author:
Siyuan JIA
;
Xiangdong LUO
;
Jie QI
;
Yongyue SU
;
Yu CHEN
;
- Publication Type:Journal Article
- Keywords:
inhibitor of differentiation;
yeast two hybrid;
Fyn
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the protein interacting with inhibitor of differentiation1′(Id1′). Methods The recombinant bait plasmid pHybLex/Zeo Id1′ was constructed and transformed into yeast strain EGY48/ pSH18 34 to test pHybLex/Zeo Id1′ for non specific activation. Adult human lung cDNA libraries were screened to obtain true positive library plasmid. The true positive library clone was obtained by sequencing and basic local alignment sequence tool (BLAST). Results The recombinant bait vector, named as pHybLex/Zeo Id1′, was confirmed by sequencing. pHybLex/Zeo Id1′ was transformed into yeast strain EGY48/pSH18 34 and the transformants had no autonomously activated reporter genes. One true positive clone, obtained by screening of the adult human lung cDNA libraries, was confirmed to be Fyn by sequencing and BLAST. Conclusion Id1′ can interact with Fyn.
