Roles of PKC in LPS-induced NF-?B activation of macrophages in rats
- VernacularTitle:PKC在LPS激活大鼠肺巨噬细胞核转录因子-?B中的作用
- Author:
Shu LI
;
Zhengqing WANG
;
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
NF ?B;
PKC;
signal transduction
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the roles of protein kinase C (PKC) in the signal transduction pathway of LPS induced nuclear transcription factor (NF) ?B activation of alveolar macrophages(AMs). Methods NF ?B level in nuclear protein extraction of AMs was detected by sandwich ELISA. Intranuclear translocation of NF ?B was observed by immunocytochemical staining. Results LPS could induce NF ?B activation of AMs in time and dose dependent manners. Immunohistochemical staining revealed intranuclear translocation following LPS induction. Specific calphostin C (Cal C) and pyrrolidine dithiocarbamate (PDTC) could inhibit LPS induced NF ?B activation. Conclusion PKC, as a up stream messenger, is involved in the signal transduction pathway of LPS induced NF ?B activation.
