Changes of biological functions of dipeptide transporter in severe burned rats and regulation of growth hormone
- VernacularTitle:严重烫伤大鼠小肠上皮细胞PepT1生物学功能的改变及生长激素的调控
- Author:
Bingwei SUN
;
Ning LI
;
Guangji WANG
;
Xiaochen ZHAO
;
- Publication Type:Journal Article
- Keywords:
Burn;
Dipeptide transporter;
Uptake;
Transport;
Regulation
- From:
Parenteral & Enteral Nutrition
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To determine the regulation effects of recombinant human growth hormone(rhGH) on dipeptide transport(PepT1) in normal and severe scalded rats. Methods: Male Sprague Dawley rats inflicted by 30% total body surface area ( TBSA) Ⅲ degree scalding were employed as the model.The rhGH was used in this study with the dose of 2 U/(kg?d). An everted sleeve of intestine was incubated in Kreb’s solution with radioactive dipeptide ( 3 H Glycylsarcosine, 3 H Gly Sar, 10 ?Ci / ml) at 37℃ for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scalded rats. Results: The transport of dipeptide in normal rats with injection of rhGH was not significantly increased compared with controls ( P =0.192 6) while the uptake was significantly increased compared with controls ( P = 0.025 3 ). The transport and uptake of PepT1 in scalded rats with injection of rhGH were significantly increased compared with controls( P = 0.008 2 and 0.039 1). Conclusions: The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells with severe injury were markedly upregulated by rhGH.
