Hypoxia inducible factor-1? expression in myocardial tissues of rats following severe burn injury
- VernacularTitle:烫伤诱导大鼠心肌细胞缺氧诱导因子-1?定位表达的研究
- Author:
Yongming DANG
;
Yuesheng HUANG
;
Lifeng CHEN
;
- Publication Type:Journal Article
- Keywords:
burn;
myocardial injury;
hypoxia induction factor 1?;
Western blot;
immunohistochemistry
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes and roles of hypoxia inducible factor 1?(HIF 1?) expression in myocardial tissues in rats following severe scald. Methods Male Wistar rats inflicted with 40% TBSA Ⅲ degree scald were used as animal models. HIF 1? protein in myocardial tissues was detected by Western blot and immunohistochemical technique. Results HIF 1? protein expression in rat myocardial tissues increased significantly in the early stage following scald. The difference in HIF 1? level between the left and right ventricles was significant. The increased HIF 1? protein was mainly located in the nucleus. Conclusion HIF 1? protein expression in myocardial tissues of rats can be induced by severe scald and HIF 1? protein expression in the left ventricle is significantly higher than that in the right ventricle. The increased HIF 1? protein in the nucleus can induce downstream cytokine expression.
