An experimental study of glucose transporter 1 protein expression in the burned rat liver
- VernacularTitle:严重烧伤大鼠肝脏葡萄糖转运体1蛋白表达的实验研究
- Author:
Huabing QI
;
Fengjun WANG
;
Shiliang WANG
;
- Publication Type:Journal Article
- Keywords:
burn;
glucose metabolism;
glucose transporter 1;
liver;
hypoxia
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of glucose transporter 1(GLUT 1) and its transcription activity in the liver of burned rats Methods The Wistar rats inflicted with 30% TBSA full thickness flame burn on the back were sacrificed at 0 5, 1, 2, 4, 8 and 16 h after burn GLUT 1 protein levels in the rat livers were determined by Western Blotting with the reference to those in 6 normal rats The liver cells were transfected with Construct A, and 24 h later subjected to hypoxia (1%O 2) to mimic the hypoxia environment The samples were harvested at 3, 6 and 12 h and determination of the reporter gene luciferase and pSV ? galactosidase activities was performed Results ①Compared with that in the normal control, GLUT 1 protein level in the liver was significantly increased( P
