Changes of NGF mRNA expression and effect of exogenous IL-1? on it in rat after traumatic brain injury
- VernacularTitle:大鼠创伤性脑损伤后NGF mRNA表达变化及外源性IL-1?对其影响
- Author:
Zheng ZHOU
;
Huisun CHEN
;
Kecheng ZHANG
;
Hui YANG
;
- Publication Type:Journal Article
- Keywords:
TBI;
NGF mRNA;
interleukin 1?
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of NGF mRNA expression and the effect of exogenous interleukin 1?(IL 1?) on it in rats after traumatic brain injury(TBI) and to explore the mechanism of NGF and IL 1? in TBI. Methods A brain trauma model of fluid percussion in rats was established. Changes of NGF mRNA expression and effect of exogenous IL 1? on it were observed by RT PCR, molecular hybridization and immunocytochemical techniques. Results NGF mRNA expression in the brain injury site and tissues adjacent to it began to increase at 12 h after trauma and increased markedly at 24 h and reached the peak value on the 3rd. Then it decreased gradually, but still higher than that of the control. NGF mRNA expression increased at 3 h after trauma in IL 1? treatment group and was significantly higher than that in simple trauma and control groups( P
