Studies on the antioxidant effect of diterpenoid compounds
- VernacularTitle:几种香茶菜属二萜类化合物的抗氧化作用
- Author:
Jun WANG
;
Shiwen GE
;
Yi WANG
;
Qixia YE
;
Tanmu ZHANG
;
- Publication Type:Journal Article
- Keywords:
diterpenoid compounds;
glutathione;
antioxidant effect;
hydroxyl radical;
superoxide anion;
nitric oxide
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the scavenging free radicals and antioxidant effect of diterpenoid compounds. METHODS Reductive cytochrome C (cytc Ⅱ) in fenton reaction system was detected by spectrophotometer to indicate the scavenging capability of the test compounds scavenging. The NO - 2 content was measured by spectrophotometery. DNTB method was used to measure the content of GSH. RESULTS oridonin (Orid), ponicidin (Pon), amethystonal(Ame), Isodonoiol(Iso) 50,100,200 ?mol?L -1 could scavenge hydroxyl radicals(?OH) in Fenten's reaction. Orid, Pon, Ame, Iso 400, 800 ?mol?L -1 could scavenge superoxide anion(O -? 2) in xanthine/xanthine oxidase system. Orid, Pon, Ame, Iso 2,4 ?mol?L -1 decreased the content of NO - 2, in macrophages stimulated by LPS and also inhibited the decrease of GSH content in liver cell caused by CCl 4. CONCLUSION Orid, Pon, Ame, Iso all have antioxidative effect through scavenging ?OH, NO - 2 and inhibiting lipid peroxidation.
