Protective effects and mechanisms of propofol on anoxia injury in cultured rat hippocampal neurons
- VernacularTitle:异丙酚对神经元缺氧损伤的保护及其作用机制
- Author:
Xiaohui QIN
;
Weidong MI
;
Hong ZHANG
;
Nan LI
;
Sheng YANG
;
- Publication Type:Journal Article
- Keywords:
hippocampal neurons;
anoxia;
calcium;
free radical;
mitochondrial membrane potential;
propofol
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective effects and mechanism of propofol on cultured rat hippocampal neurons subjected to anoxia injury METHODS Hippocampal neurons of neonatal rats, which had been cultured in vitro for 10 days, were allocated to control groups and propofol treating groups In propofol treated groups,the culture medium were loaded with propofol at the concentrations of 3, 6, 12, 24, 48 mg?L -1 respectively, and then the neurons were exposed to oxygen glucose deprivation for 24 h, to 40 ?mol?L -1 H 2O 2 for 24 h or to 100 ?mol?L -1 glutamate for 50 min The cell survival rate in each group was evaluated by MTT colorimetry. Using a laser scanning confocal microscope (LSCM), the effects of propofol on neuronal calcium overload and on the reduction of mitochondrial membrane potential (△?m) evoked by anoxia were observed with fluo 3 and rhodamine 123 for real time changes of [Ca 2+ ] i and △?m. The electron spin resonance (ESR) was used to measure the scavenging effects of propofol on hydroxyl radical and superoxide anion RESULTS Propofol at the concentrations of 6～48 mg?L -1 attenuated the anoxic injury ( P
