Accelerating effects of high glucose on NE-induced cardiac hypertrophy of the cultured myocardial cells
- VernacularTitle:高糖对去甲肾上腺素诱导的心肌细胞肥大的促进作用
- Author:
Qingfeng ZHOU
;
Hongxin WANG
;
Guijun WANG
;
Yuhong YANG
;
- Publication Type:Journal Article
- Keywords:
high glucose;
cardiac hypertrophy;
norepinephrine;
diabetic cardiomyopathy
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of high glucose on the cardiac hypertrophy,cultured cardiomyocytes were used to study the accelerating effects of high glucose on norepinephrine(NE) induced cardiac hypertrophy. METHODS Using cultured myocardial cells as a model, the cellular hypertrophy was observed. The protein content was assayed with Lowrys method. The cardiomyocytes' volumes was measured by computer photograph analysis system. The protein synthesis was assayed with leucine intake method. RESULTS The total cellular protein content?cellular volumes?cellular protein synthesis showed an increase in high glucose group, NE group, high glucose and NE group compared with control group, respectively. High glucose and NE group showed a further increase compared with NE group. CONCLUSIONS The high glucose itself induced hypertrophy of the cultured myocardial cells slightly. Meanwhile high glucose can further accelerate hypertrophy of the cultured myocardial cells induced with NE. The effective mechanism between high glucose and NE on cardiac hypertrophy need further study.
