Tripterygium Hypoglaucum (Levl) Hutch alkaloids induce DNA strand breaks and DNA fragmentation in Jurkat T lymphoma cells
- VernacularTitle:昆明山海棠碱诱导Jurkat淋巴瘤细胞核DNA链断裂和DNA片段化
- Author:
Lujun YANG
;
Michael NUESSE
;
Lin AO
;
Jia CAO
;
- Publication Type:Journal Article
- Keywords:
Tripterygium Hypoglaucum (Levl) Hutch;
apoptosis;
DNA content;
DNA strand breaks;
TUNEL
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Tripterygium Hypoglaucum (Levl) Hutch alkaloids on the nuclear DNA strand breaks and DNA fragmantation in Jurkat T lymphoma cells to understand the mechanisms of the apoptosis. Methods After Jurkat cells were induced by the alkaloids, DNA stand breaks were labeled by TUNEL assay and DNA fragmentation was analyzed by DNA content analysis. Flow cytometry was performed to determine these phenomena. Results THH alkaloids could effectively induce DNA stand breaks and DNA fragmentation. Conclusion There are great changes in nuclear DNA in the apoptosis of Jurkat T lymphoma cells induced by THH alkaloids.
