Determination of host bacterium genomic DNA content in DNA vaccine with digoxigenin-labeled probe
- VernacularTitle:地高辛标记的DNA探针检测核酸疫苗中宿主菌基因组DNA的含量
- Author:
Yan HE
;
Ke SHI
;
Shuhan SUN
;
- Publication Type:Journal Article
- Keywords:
digoxigenin;
DNA probes;
vaccine, DNA
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
The ribosomal DNA fragment was amplified with PCR utilizing synthetic primers with host bacteria chromosomal DNA as template. The resultant probes were labeled with Digoxigenin and were applied in the dot blot of DNA vaccine samples. The host bacteria genomic DNA in DNA vaccine against hepatitis B was less than 15 ng/?g. Digoxigenin labeled probes proved sensitive and reliable in determining genomic DNA.
