Association of disease progression with dynamic alterations of clinical immunological and virological factors in patients with severe acute respiratory syndrome
- VernacularTitle:严重急性呼吸综合征患者的临床免疫学和病毒学变化特点及其相关致病机制
- Author:
Yonggang LI
;
Fusheng WANG
;
Min WANG
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Severe acute respiratory syndreme;
Immunolog;
Virologig
- From:
Chinese Journal of Infectious Diseases
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Background to investigate the features of clinical immunological and virological factors during the SARS disease progression and therapeutic process and evaluate their implications in diagnosed SARS patients. Methods Thirty three patients with diagnosed SARS were recruited in the study and 25 healthy subjects were employed as a control. The SARS virus S gene were amplified by using the PCR assay and direct DNA sequencing. The dynamic alterations of circulating T lymphocyte subsets and dendritic cellular subsets were determined by flow cytometric analyses. The plasma SARS antibodies were evaluated using ELISA assay. Their implications in disease progression of SARS were evaluated based on the clinical data. Results The peripheral CD4 + T lymphocytes, CD8 + T lymphocytes, NK cells, and dendritic cell subsets were compared with healthy subjects and found to be significantly lower in progressive SARS patients. In particular, the dynamic alteration of the circulating,T lymphocyte subsets, and DC subsets was found to undergo a dynamic alteration of from high level to lower level, gradually recover to normal level during the initiation stage, progressive and convalescence stage, respectively, in SARS patients. Furthermore, the plasma positive rates were around 30% for SARS viral gene detections in early stage in SARS patients and the plasma positive rates for both anti SARS IgM and IgG antibodies went up to peak at seventh week after onset of SARS diseases. Conclusions SARS coronavirus infection results in the significant decrease of peripheral blood T lymphocyte subsets and dendritic cell subsets, which correlates with the SARS natural disease process in patients. Our results would help to understand the pathogenesis of SARS virus infection as well as for evaluation of therapeutic efficacy and clinical outcome of SARS patients.
