Cloning and expression of the human MMP-1 gene fragment and antigenecity analysis of its expression products
- VernacularTitle:人基质金属蛋白酶-1的基因克隆、表达及其产物抗原性的分析
- Author:
Jue HOU
;
Guoling HU
;
Shuanghu LIU
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Human MMP 1;
Clone;
Prokaryotic expression;
Antigenecity
- From:
Chinese Journal of Infectious Diseases
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the recombinant plasmid of human MMP 1 clone and study its antigenecity of MMP 1 fusion protein. Methods The total RNA was extracted from human liver and used as a template for reverse transcription. After PCR amplification, a 1 432 bp fragment was obtained and cloned into T vector. After digested with restriction enzyme, the target fragment was subcloned into plasmid pMAL c2x. The recombinant plasmid was transferred into JM109 which can express a fusion protein. We analyze the protein with SDS PAGE and Western blot. Results We obtained human MMP 1 gene and its recombinant plasmid clone. The expressed protein can be recognized by MMP 1 polyclonal antibody in Western blot.Conclusions We obtained the MMP 1 protein with antigenicity. The fusion protein can be used to prepare polyclonal antibody against MMP 1.
