Effect of noggin gene on proliferation of neural precursor cells from the adult rat hippocampus
- VernacularTitle:Noggin基因对成年大鼠海马神经前体细胞增殖的影响
- Author:
Xiaotang FAN
;
Wenqin CAI
;
Haiwei XU
;
Zhong YANG
;
Jinhai ZHANG
;
- Publication Type:Journal Article
- Keywords:
noggin;
hippocampus;
bromodeoxyuridine;
bone morphogenetic protein 4;
rat
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of noggin on BrdU labeled cells in the adult rat hippocampus. Methods The expressions of noggin and bone morphogenetic protein 4 (BMP4) in rat hippocampus were detected using in situ hybridization histochemistry (ISHH) and reverse transcription polymerase chain reaction (RT PCR). By using antisense technique combined with bromodeoxyuridine!(BrdU) labeling, the effect of noggin on hippocampal neurogenesis in adult rats was explored. Results The number of noggin mRNA positive cells in the adult rat hippocampus decreased significantly after treatment with antisense noggin but no change was found in the number of BMP4 mRNA positive cells. In addition, the number of BrdU labeled cells decreased significantly in the adult rat hippocampus after treatment with antisense noggin, but the sense noggin had no such effect. Conclusion Noggin can promote proliferation of neural precursor cells in adult rat hippocampus.
