Roles of uPA and uPA-R in the course of repair after human embryo corneal alkali burn in vitro
- VernacularTitle:uPA和uPA-R在人胚胎角膜碱烧伤后修复过程中作用的体外研究
- Author:
Jun YAN
;
Tian YANG
;
Guoping LI
;
Qiangguo GAO
;
Jin YANG
;
- Publication Type:Journal Article
- Keywords:
urokinase type PA;
uPA receptor;
human embryo cornea;
alkali burn;
repair
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the roles of urokinase type PA (uPA) and uPA receptor (uPA R) in the course of repair after human embryo corneal alkali burn in vitro . Methods After the alkali burn model in vitro was established successfully, some techniques such as the observation of cell culture and morphology, ICC and image analysis were used. Results No significant difference was found between the cells from corneal limbus and central cornea and almost all of them expressed AE1/AE3. The expressions of uPA and uPA R increased to the maximum at about 24 h after alkali burn. uPA expression distributed mainly in the cytoplasm but uPA R expression distributed mainly in the cell membrane. Conclusion The corneal epithelial cells of comparatively high purity can be acquired by tissue culture. There probably exists difference in development and vitality between embryo and adult cornea. There might be commonness of the roles of uPA and uPA R in epithelial tissue.
