Effects of cholesterol-inhibitor lovastatin on the activity of MAPK in MCF-7 breast cancer cells
- VernacularTitle:胆固醇合成抑制剂洛伐他汀对MCF-7乳腺癌细胞MAPK活性的影响
- Author:
Na WEI
;
Mantian MI
;
Jundong ZHU
;
Haibin LANG
;
- Publication Type:Journal Article
- Keywords:
lovastatin;
MCF 7 cell;
protein expression;
phosphorylation levels;
MAPK activity
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lovastatin on MAPK activity in MCF 7 breast cancer cells. Methods After MCF 7 cells were treated with 4, 8 and 16 ?mol/L lovastatin for 48～72 h, the expressions of ERK1 and p38 MAPK proteins were detected by Western blotting. Their phosphorylation levels were observed by kinase activity assay. Results MCF 7 cells treated with different doses of lovastatin for 48～72 h had no significant effect on the protein level of ERK1, but the activities of ERK1 and the P38 MAPK proteins were down regulated at 72 h after treatment. Conclusion Lovastatin may down regulate the activity of MAPK in MCF 7 cells by means of inhibiting the mevalonate metabolism pathway and/or affecting phosphorylation levels.
