In vitro amplification of immortalized chondrocytes with micro-carriers in RCCS
- VernacularTitle:永生化软骨细胞体外快速扩增的实验研究
- Author:
Zeqiang FANG
;
Changyong WANG
;
Huizeng LI
;
Yuan SUN
;
Jun YANG
;
- Publication Type:Journal Article
- Keywords:
immortalization;
cartilage tissue engineering;
rotary cell culture system;
microcarrier
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method and technique to gain adequate seed cells for cartilage tissue engineering Methods hTERT gene was introduced into rabbit mandibular condylar chondrocytes by eukaryotic vector Rapidly proliferated immortalized chondrocytes in positive clones in micro carrier rotary cell culture system (RCCS) were screened and selected The growth of immortalized chondrocytes and the metabolic rate were observed Collagen type Ⅱ expression of immortalized chondrocytes of the experimental groups was observed The immunohistochemical results were compared with those of the control groups Results The immortalized chondrocytes in experimental groups could grow rapidly with a high metabolic rate and shorter population doubling time (PD) ( P
