Effects of quercetin on metabolism of cyclooxygenase in IEC-6 cells stimulated by the serum of burned rats
- VernacularTitle:槲皮素对烫伤大鼠血清刺激的IEC-6细胞环氧合酶活性及代谢产物的影响
- Author:
Desheng MENG
;
Fengjun WANG
;
Shiliang WANG
;
- Publication Type:Journal Article
- Keywords:
quercetin;
burn;
IEC 6;
PGE 2;
cyclooxygenase
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of quercetin on PGE 2 release and cyclooxygenase activity in IEC 6 cells stimulated by the serum of burned rats. Methods The PGE 2 levels were measured by radioimmunoassay and the changes of cyclooxygenase activity were determined by substrate fluorescence analysis. Results Stimulated by the serum of burned rats, the PGE 2 level increased first and then obviously decreased. Quercetin at 0.1 and 1.0 ?mol/L enhanced the PGE 2 level after the stimulation of IEC 6 cells for 24 h. There was no obvious change in total cyclooxygenase activity, but the activity ratio of COX 1 to COX 2 significantly decreased. Conclusion Quercetin might influence the release of cyclooxygenase metabolite PGE 2 through the regulation of COXs activity in IEC 6 cells.
