Changes of apoptosis and caspase-3 activity and the effects of Ac-DEVD-CHO in cardiomyocytes of rats with myocardial reperfusion injury
- VernacularTitle:大鼠心肌缺血再灌注时心肌细胞凋亡caspase-3活性变化规律及药物对其影响
- Author:
Qiang XU
;
Liangyi SI
;
Xiaolan ZHAO
;
Zhang WANG
;
- Publication Type:Journal Article
- Keywords:
myocardial reperfusion injury;
apoptosis;
caspase 3
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of apoptosis and caspase 3 activity in cardiomyocytes of rats at different time points after transient ischemia and the effects of caspase 3 inhibitor Ac DEVD CHO on reperfusion injury Methods A total of 122 rats were divided randomly into 3 groups: IR group, IR+Ac DEVD CHO group and sham group The ischemic samples were observed at 30 min after ischemia followed by reperfusion at 1, 3, 6, 12 and 24 h The apoptotic cardiomyocytes, the activity of caspase 3 and the myocardial infarction area were determined by TUNEL method, fluorescent assay and TTC dyeing method, respectively Results Apoptosis index(AI) of cardiomyocyte and caspase 3 activity changed in a time dependent manner Highest AI and caspase 3 were found after reperfusion at 12 h The myocardial infarction area in group IR kept increasing during the period of reperfusion AI and caspase 3 activity were positively correlated with the myocardial infarction area( P
