Stability of recombinant human epidermal growth factor in artificial intestinal juice and gastric juice
- VernacularTitle:重组人表皮生长因子在人工胃液和肠液中的稳定性
- Author:
Shengying YE
;
Shiling WANG
;
Shen GAO
;
- Publication Type:Journal Article
- Keywords:
recombinant human epidermal growth factor;
artificial intestinal juice;
artificial gastric juice;
stability;
peptic enzymes
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper is to study the stability of recombinant human epidermal growth factor (rhEGF) in artificial intestinal juice and gastric juice,providing references for its clinical application in intestinal and stomach injury.RhEGF was added into artificial intestinal juice and gastric juice respectively, and the content of rhEGF was determined with RIA at preset time points. The content of rhEGF in artificial gastric juice (pH 2) and intestinal juice (pH 6.8) had a tendency to decrease as time went by,reaching 43.46% and 21.91% from their baselines 1 h later respectively. But the decrease of rhEGF reached 89.62% and 79.52% 1 h after the presence of peptic enzymes respectively. The rhEGF in the artificial intestinal juice and gastric juice was relatively stable but the process of its inactivation could be greatly expedited in the presence of peptic enzymes.
