Interventional effect of tetrapeptide on sulfur mustard-induced lymphocyte apoptosis
- VernacularTitle:四肽AC-DEVD-CHO对硫芥诱导淋巴细胞凋亡的干预作用
- Author:
Yan SAI
;
Yong LIU
;
Mingxue ZHU
;
Liming ZHANG
;
Zhaojun DONG
;
- Publication Type:Journal Article
- Keywords:
tetrapeptide;
sulfur mustard;
apoptosis;
caspase 3
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of caspase 3 activity and the interventional effect of tetrapeptide AC DEVD CHO on sulfur mustard induced apoptosis of lymphocyte. Methods The activity of caspase 3 was detected by fluorospectrophotometry. DNA ladder and apoptosis were detected by DNA agarose gel electrophoresis and flow cytometry. Results Sulfur mustard enhanced the activity of caspase 3 and its polypeptide inhibitor (AC DEVD CHO) partially blocked apoptosis, which was mainly presented as an obscure phenomenon of DNA ladder and a decrease in percentage of apoptosis. Conclusion Sulfur mustard can induce the apoptosis of lymphocyte by means of activating caspase 3. The inhibitor may have an interventional effect on lymphocyte apoptosis induced by sulfur mustard.
