Construction and application of a fusion expression vector containing subunit B of Escherichia coli heat-labile enterotoxin
- VernacularTitle:大肠杆菌不耐热肠毒素B亚单位融合表达载体的构建及应用
- Author:
Dongshui LU
;
Xuhu MAO
;
Chao WU
;
Quanming ZOU
;
- Publication Type:Journal Article
- Keywords:
subunit B of heat labile enterotoxin;
fusion expression;
vector
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a fusion expression vector with subunit B of Escherichia coli heat labile enterotoxin. Methods Gene encoding LTB without stop codon obtained by PCR was introduced to a linker and was recombined on vector Pinpoint Xa I through Eco RⅠ and Eco RⅤ sites and then fused with ureB. The recombinant was used to transform E. coli JM109. The expression of LTB was analyzed by SDS PAGE and Western blotting. Results The fusion expression plasmid was successfully constructed. The subunit B of urease of Helicobacter pylori (Hp) fusogenic protein with LTB with the ability to bind GM 1 and the reactogenicity with polyclonal antibodies against Hp was harvested. Conclusion The successfully constructed vector provides experimental base for the studies of intramolecular adjuvant vaccine.
