Establishment and application of xenopus leavis egg cell-free translating system
- VernacularTitle:非洲爪蟾卵非细胞翻译体系的建立及应用
- Author:
Huikai TIAN
;
Xiaohao YAO
;
Bing MA
;
Hongmei ZHANG
;
Xuejun LI
;
- Publication Type:Journal Article
- Keywords:
cell free translation system;
apoptosis;
TFAR19;
Xenopus leavis egg
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an efficient and convenient system for expressing functional proteins. METHODS Xenopus leavis females were injected with HCG, eggs were harvested next morning and dejellied by 2% cysteine, from which the Xenopus leavis egg cell free translating system was prepared. Through subcloning technique, TFAR19 gene was cloned to pBluescript SK plasmid which was linearized by enzyme, then transcript and capped in vitro to synthesis the cRNA of TFAR19. RESULTS This cRNA was translated in freshly prepared cell free translating system, the product was identified by Western Blot. CONCLUSION The results showed that this translating system could efficiently translate TFAR19 cRNA and be a very useful translating tool.
