Anti-tumor activity of extracts from cultured cells of Taxus chinensis on cancer cell lines SMMC-7721 and HEp-2
- VernacularTitle:红豆杉细胞提取物对肿瘤细胞SMMC-7721和HEp-2的作用研究
- Author:
Cuitao LU
;
Xingguo MEI
;
Wei GONG
;
Yan LIU
;
- Publication Type:Journal Article
- Keywords:
Taxus chinensis;
callus cell;
SMMC 7721;
HEp 2;
cell toxicity;
cell cycle
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze the toxicity and inhibitory mechanism of extracts from cultured cells (F 4 cell line) of Taxus chinensis on cancer cell lines SMMC 7721 and HEp 2. METHODS MTT assay for cell viability and flow cytometry for cell cycle analysis. RESULTS IC 50 of SMMC 7721 and HEp 2 were 0 161 4 g DCW?L -1 and 0 275 6 g DCW?L -1 respectively,tumor cells in G 2～M stage all increased with higher concentration and longer incubation of extracts from Taxus chinensis cells. CONCLUSION Extracts from cultured cells of Taxus chinensis could have cytotoxic effect on SMMC 7721 and HEp 2 and could induce apoptosis of both two cancer cells.
