Changes of NO content and its cell source in rat brain at the early period following ischemic injury
- VernacularTitle:大鼠缺血性脑损伤早期一氧化氮含量的变化及其细胞来源的研究
- Author:
Hongli LI
;
Wenqin CAI
;
Shifu ZHAO
;
Yu SUN
;
- Publication Type:Journal Article
- Keywords:
cerebral ischemia/reperfusion;
nitric oxide;
glial fibrillary acidic protein;
rats
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of nitric oxide(NO) content and explore its cell source following brain ischemic injury. Methods We established the model of transient global brain ischemia/reperfusion (IR) in rat. The concentration of NO and its cell source were investigated by detection of NO content, NDP histochemical staining, double label technique of immunofluorescence and stereological analysis after brain ischemia. Results (1)The content of NO increased at 12 h and peaked on days 1～3 after IR. The content of NO decreased gradually on the 5th day after IR. (2)The density of NDP positive cells increased and peaked on the 1st day after IR. The positive cells distributed mainly in the temporal cortex, hippocampus and periventricular zone after IR. The positive cells were found to reduce gradually or disappear on the 14th day after IR. (3)Few NDP positive and GFAP immunofluorescence positive cells were found to coexist at the early period of IR(1～3 d). The percentage of coexisting cells was 10%～15%. Conclusion The content of NO increases at the early period after ischemic brain injury. The main cell type to produce NO is neuron.
