Regulation of the extracellular matrix synthesis and degradation in liver fibrotic rats by antisense TIMP_1
- VernacularTitle:反义TIMP_1基因对纤维化肝脏间质胶原合成的影响
- Author:
Zhong ZENG
;
Benli HAN
;
Hengchun DUAN
;
- Publication Type:Journal Article
- Keywords:
liver fibrosis;
interstitial collagen;
antisense TIMP 1 gene
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of antisense TIMP 1 on the interstitial collagen synthesis of fibrotic liver in rats. Methods Fibrosis was induced in the liver of rats with the injection of 60% CCl 4 and 5% alcohol. Recombinant antisense TIMP 1 gene mammalian expression vectors were construct and infused into the fibrotic liver of rats. The expressions of TIMP 1 mRNA, ?1(Ⅲ) mRNA and protein were determined by RT PCR, ISH and immunohistochemistry. The serum levels of PCⅠ, PCⅢ in rats were determined by RIA. Results Antisense TIMP 1 gene could markedly suppress the expression of endogenous TIMP 1 mRNA in the transfected liver of rats( P
