Polypeptides based on CTL, T helper and B-epitopes of HBV antigens induce HLA-A2~+ human PBMCs to produce CD8~+ CTL-mediated cytotoxicity
- VernacularTitle:多表位组合肽诱导HLA-A2~+人PBMC产生抗原特异性CD8~+T细胞应答的研究
- Author:
Tongdong SHI
;
Yuzhang WU
;
Wei ZHOU
;
Zhengcai JIA
;
Liyun ZOU
;
- Publication Type:Journal Article
- Keywords:
hepatitis beta virus;
cytotoxic T lymphocytes;
therapeutic peptide;
epitope;
molecular design
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore how to trigger an HLA Ⅰ restricted CD8 + T cell response to exogenously synthesized peptides in vitro . Methods A new panel of therapeutic peptides based on the immunodominant B and CTL epitopes of HBV PreS 2 region and HBcAg and the tetanus toxoid common T helper epitopes were synthesized by Merrifield solid phase peptide synthesis, and HLA A2 + human PBMCs were used to investigate the immunological properties of the mimetic peptides. Results The results demonstrated that the peptides could trigger vigorous CD8 + HBV specific CTL responses in vitro specifically and effectively. Conclusion The results reveal that T helper plus B epitopes designing with the introduction of short and flexible linker can remarkably improve the immunogenicity of short peptides and hence produce effective CTL responses in vitro .
