Transfection and expression of Smad7 inhibits transforming growth factor-?_1 effects on renal tubular cells
- VernacularTitle:Smad7基因转染拮抗TGF-?_1对培养肾小管细胞的影响
- Author:
Yunjian HUANG
;
Yuming MEI
;
Yiqin WANG
;
Tangjun YANG
;
- Publication Type:Journal Article
- Keywords:
Smad7;
TGF ? 1;
gene transfection;
renal cells;
cell cycle
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Smad7 gene transfer on tubular cell cycle arrest, tubular apoptosis and fibronectin (FN) synthesis by transforming growth factor ? 1(TGF ? 1) induced. Methods Mouse Smad7 gene was transfected into renal tubular cells in primary cell culture by using Tfx 50 cationic liposome. Tubular cell proliferation was measured by MTT and cell cycle was observed by flow cytometry. The level of FN secretion in the supernatant was determined by ELISA. Results At 48 h after administration of 10 ng/ml TGF ? 1 to renal tubular cells, cell proliferation declined, G 0/G 1 arrested in cell cycle, and cell FN secretion increased significantly. These abnormalities were attenuated by liposome mediated Smad7 gene transfection. Conclusion Transfection and expression of Smad7 can markedly inhibit TGF ? 1 responses in renal tubular cells, which is helpful for further studies of Smad7 gene therapy in vivo .
