Effects of cyclooxygenase-2 and its specific inhibitor NS-398 on the growth and invasion ability of urothelial carcinoma cell line EJ
- VernacularTitle:环氧合酶-2及其特异性抑制剂NS-398对膀胱癌细胞体外生长与侵袭能力的影响
- Author:
Guoping LI
;
Tian YANG
;
Jintao LI
;
Jin YU
;
Jun YAN
;
- Publication Type:Journal Article
- Keywords:
cyclooxygenase 2;
urothelial carcinoma cell line EJ;
carcinoma;
invasion;
uPA;
NS 398
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of cyclooxygenase 2(COX 2) and its specific inhibitor NS 398 on the cell growth and invasion ability of urothelial carcinoma cell line EJ. Methods The cox 2 cDNA was transfected into the urothelial carcinoma cell line EJ and a cell line EJ COX 2 which highly expressed cox 2 gene permanently was gained. The cell growth rate before and after transfection was observed. Then at various concentrations of NS 398, the invasion ability was detected by Boyden Chamber and expression levels of uPA by RT PCR and Western blot. Results The EJ COX 2 cell line grew more rapidly and had a stronger invasion ability than EJ and its uPA expression increased significantly. NS 398 could dose dependently inhibit the expressions of COX 2 and uPA and the invasiveness of EJ COX 2 cell. Conclusion COX 2 can stimulate the growth of urothelial cell line EJ and promote its invasion ability by stimulating the expression of uPA.
