Construction of cDNA library from adults of Anopheles dirus
- VernacularTitle:大劣按蚊成蚊cDNA文库的构建与质量鉴定
- Author:
Hongxing HAO
;
Wenyue XU
;
Jianhua DUAN
;
Mingshu KUANG
;
Fusheng HUANG
;
- Publication Type:Journal Article
- Keywords:
cDNA library;
Anopheles dirus
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct cDNA library from adults of Anopheles dirus for cloning the immune genes or related genes for malaria parasites development. Methods The mRNA of adult Anopheles dirus was isolated. The library was constructed by using the Zap Express vector(Stratagene) and the quality was evaluated. Results The efficiency of the library was 2.1?10 6 pfu/ml with 98% clones positive. The average length of the insert fragment was over 1 kb. Conclusion cDNA library of adult Anopheles dirus with high efficiency can be constructed by using the Zap Express library construction Kit (Stratagene).
