Study of the related influencing factors of MCF-7 cells proliferation test in vitro
- VernacularTitle:影响MCF-7细胞增殖试验相关因素的初步研究
- Author:
Yi ZHU
;
Weiqun SHU
;
Huaijun TIAN
;
- Publication Type:Journal Article
- Keywords:
environmental estrogen (EE);
MCF 7cells;
cell proliferation test
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the related influencing factors of MCF 7 cells proliferation test in vitro to provide some criteria for the standard detection of environmental estrogens (EE) by cell proliferation test of MCF 7 cells in vitro . Methods The sensitivity to E 2 stimulation of different sources of MCF 7 cell lines was detected. Cell proliferation test was performed on the screened sensitive cells in different culture conditions. Results In the estrogen free medium, C cell line of MCF 7 was more sensitive to E 2 stimulation than A and B cell lines ( P
