Effect of sodium butyrate on the growth and differentiation of human glioma cell line SHG-44
- VernacularTitle:丁酸钠对人脑胶质瘤细胞系SHG-44增殖分化的影响
- Author:
Yan ZHAN
;
Xinggang ZHI
;
- Publication Type:Journal Article
- Keywords:
butyrate;
glioma;
induced differentiation
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sodium butyrate on the growth suppression and cellular differentiation of cultured SHG 44 glioma cell line Methods Human glioma SHG 44 cells were treated with 2 mmol/L sodium butyrate The inhibition of cell growth was assessed by MTT spectrophotometric analysis and AgNORs analysis The cellular differentiation was determined by flow cytometry, electron microscopy, immunohistochemical method and agglutination test Results Treatment of SHG 44 cells with sodium butyrate resulted in significant inhibition of cell growth and G1 S transition in time dependent and dose dependent manners Sodium butyrate could induce differentiation of SHG 44 cells with regularized nuclei and increased heterochromatins The mitochondria and rough endoplasmic reticulum increased in density in normal state Furthermore, SHG 44 cells expressed more GFAP but less vimentin, and the agglutination degree decreased obviously Conclusion Sodium butyrate may suppress glioma cells growth and induce differentiation of human glioma cells
