Down-regulation of the bcl-2 expression level in a human cholangiocarcinoma cell line using ribozyme
- VernacularTitle:利用核酶技术下调人胆管癌细胞bcl-2表达水平
- Author:
Yudong FAN
;
Kaizong LI
;
Yongtong ZHAO
;
- Publication Type:Journal Article
- Keywords:
ribozyme;
cholangiocarcinoma;
bcl 2
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of down regulation of bcl 2 expression of human cholangiocarcinoma cells with bcl 2 mRNA cleaving ribozyme (RZ). Methods Synthetic gene of bcl 2 mRNA cleaving RZ was recombined with the expression vector of eukaryotic cells. The recombined vector was used to transfect human cholangiocarcinoma cells. The expression of bcl 2 in human cholangiocarcinoma cells was observed by immunocytochemistry and flow cytometry. Results The expression of bcl 2 in human cholangiocarcinoma cells transfected with RZ gene was lower than that of control group. Spontaneous apoptosis was observed in a few of the cells. Conclusion bcl 2 mRNA cleaving RZ can down regulate the expression of bcl 2 of cholangiocarcinoma cells notably and can also induce spontaneous apoptosis.
