Construction and identification of recombinant adenovirus shuttle plasmid pAdCMVLacZ
- VernacularTitle:腺病毒穿梭质粒pAdCMVlacZ的构建及鉴定
- Author:
Ping YANG
;
Dajun YING
;
- Publication Type:Journal Article
- Keywords:
adenovirus vector;
lacZ
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct replication deficient adenovirus vector AdCMV lacZ Methods Recombinant adenovirus shuttle plasmid pAdCMVlacZ was first constructed and then lacZ cDNA cloned into Hind Ⅲ and Eco R Ⅴ of adenovirus shuttle plasmid pAdCMV by conventional methods Results The restriction enzymatic map of pAdCMVlacZ accorded with theoretic analyses and pAdCMVlacZ showed blue bacterium groups under the existence of revulsant and X gal Conclusion The adenovirus shuttle plasmid pAdCMVlacZ has been constructed successfully
