Effects of dexamethasone and N-acetylcysteine on p38 protein kinase induced by lipopolysaccharide in the alveolar macrophages
- VernacularTitle:NAC和地塞米松对脂多糖诱导大鼠肺泡巨噬细胞p38蛋白激酶的影响
- Author:
Cuiping HUANG
;
Zhenxiang ZHANG
;
Yankun GUO
;
Deling LI
;
- Publication Type:Journal Article
- Keywords:
p38 mitogen activated protein kinase;
lipopolysaccharide;
tumour necrosis factor ?;
interleukin 8
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the activation of p38 protein kinase in alveolar macrophages(AMs) stimulated with lipopolysaccharide(LPS) and the effects of dexamethasone(DEX) and N acetylcysteine(NAC) on the process. METHODS AMs isolated and purified from normal rats were divided into four groups:Control group, LPS stimulated group ,DEX group and NAC group. The activation of p38 protein kinase in nuclear protein extract from the AMs and the concentration of TNF ? and IL 8 in supernatant were measured by Western blot and radioimmunoassay, respectively. RESULTS The activation of p38 protein kinase and the concentration of TNF ?, and IL 8 in LPS stimulated group were significantly higher than those in control group( P
